Determination of Glutathione and Glutathione Disulphide in Lichens: a Comparison of Frequently Used Methods
Identifieur interne : 003469 ( Main/Exploration ); précédent : 003468; suivant : 003470Determination of Glutathione and Glutathione Disulphide in Lichens: a Comparison of Frequently Used Methods
Auteurs : Ilse Kranner [Autriche] ; Dieter Grill [Autriche]Source :
- Phytochemical Analysis [ 0958-0344 ] ; 1996-01.
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Abstract
The determination of glutathione (GSH) and glutathione disulphide (GSSG) is subject to substantial problems when analysing plants containing high amounts of phenolic compounds, since the phenolics may react with the GSH present during the extraction. Three frequently used methods for the analysis of GSH and GSSG in the lichen Pseudevernia furfuracea have been compared. Glutathione seemed to be underestimated by the enzymatic recycling assay. Determination by high‐performance liquid chromatography after derivatization of glutathione with 2,4‐dinitro‐1‐fluorobenzene (DNFB) or labelling with monobromobimane (mBBr), respectively, gave similar results. Derivatization with DNFB gave the lowest values for GSSG expressed as a percentage of total glutathione. Cyst(e)ine and γ‐glutamyl‐cyst(e)ine were only detected with the mBBr assay.
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DOI: 10.1002/(SICI)1099-1565(199601)7:1<24::AID-PCA277>3.0.CO;2-2
Affiliations:
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